Testing acc. to ISO 13365-1: Leather - Chemical determination of the preservative (TCMTB, PCMC, OPP, OIT) content in leather by liquid chromatography - Part 1: Acetonitrile extraction method
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Our test laboratory determines preservatives in leather according to DIN EN ISO 13365-1 using an accredited test method. The standard specifies the quantitative determination of the solvent-extractable total content of TCMTB (2-(thiocyanomethylthio)benzothiazole), PCMC (4-chloro-3-methylphenol), OPP (2-phenylphenol) and OIT (2-octylisothiazol-3(2H)-one). The method can also be used to determine degradation products of these preservatives.
For the analysis, the prepared leather sample is extracted with acetonitrile in an ultrasonic bath. The resulting extract is then analysed by high-performance liquid chromatography (HPLC); chromatographic detection may be performed using, for example, UV or mass spectrometric detection. The results are reported as the content of the respective preservative in mg/kg of leather.
DIN EN ISO 13365-1 is particularly suitable when the solvent-extractable total content of preservatives in leather is to be determined. If, instead, the fraction that can be released by perspiration under conditions simulating possible skin contact is to be investigated, DIN EN ISO 13365-2 provides a complementary test method using artificial perspiration extraction.
