Collagen Analysis

Collagen Analysis

The biochemical laboratory of the collagen department provides a comprehensive material characterisation "around collagen". Tests are established according to ASTM-Standard F 2212 "Characterization of Type I Collagen as Starting Material for Surgical Implants and Substrates for Tissue Engineered Medical Products (TEMPs)“ and thus meet the requirements in the framework of an approval of collagenous medical products. The spectrum of methods is continuously extended and also involves non-collagenous proteins (such as elastin or keratin) and other biomolecules of extracellular matrix (such as glycosaminoglycan). The following analysing methods are available at FILK:

  • Chemical sum parameters (collagen content, protein content, fat content, mineral content, total sugar content)

  • Amino acid analysis

  • Elastin content

  • Degree of cross-linking

  • Chromatography (HPLC, LC-MS)

  • Mass spectrometry (LC-MS/MS, MALDI-TOF/TOF)

  • Electrophoresis (SDS-PAGE, IEF)

  • Spectroscopy (UV-Vis, FTIR)

  • Microscopy (light microscopy, REM, AFM)

  • Denaturation temperature (using DSC)

  • Enzyme stability (trypsin, collagenase)

  • Rheology

  • Physical parameters (solubility, swelling, water vapour permeability)

  • Mechanical strengths (tensile force, elongation at break), also in micro- and nanoscale

  • Special analyses on request


FILK Freiberg Institute gGmbH
Meißner Ring 1-5
09599 Freiberg


Fon: +49-(0)3731-366-0
Fax: +49-(0)3731-366-130